卵巢上皮性肿瘤端粒酶活性定量检测

Significance of telomerase activity in ovarian epithelium tumor

目的:探讨端粒酶在上皮性卵巢肿瘤发生发展中的作用,评价端粒酶作为卵巢癌诊断及预后指标的价值。方法:采用端粒酶PCR-ELISA法对25例卵巢上皮性肿瘤(8例良性、3例交界性和14例恶性)和6例正常卵巢表面上皮进行端粒酶活性定量检测。结果:2例良性、2例交界性和12例恶性卵巢上皮性肿瘤及1例正常卵巢上皮存在端粒酶活性增强,8例良胜、3例交界性、14例恶性和6例正常卵巢上皮端粒酶活性吸光值的x±s分别为0.080±0.070、0.408±0．208、1.659±0．930和0.086±0.060。统计学分析表明恶性卵巢癌中端粒酶活性明显高于良性、交界性肿瘤和正常卵巢组织;端粒酶活性和卵巢上皮性肿瘤组织学类型、临床分期无关;而与肿瘤的分化程度有关。结论:研究结果初步证明端粒酶活性增强在卵巢癌的无限增殖中是一重要环节,并提示端粒酶活性增强有可能成为卵巢癌的标志物之一。

Aim: To investigate the role of telomerase activity in ovarian epithelium tumor, and to evaluate its significance in diagnosis as a tumor biomarker. Methods: Telomerase activity was measured quantitatively by Telomerase-PCR-ELISA in 25 samples of ovarian epithelium tumors (including 8 benign, 3 borderline-malignant, and 14 malignant tumors) and 6 samples of normal ovarian epithelia as control. Results: The increase of telomerase activity was detected in 2 of 8 benign, 2 of 3 borderline-malignant, 12 of 14 malignant tumor samples, and 1 of 6 cortex of normal ovaries, and the levels of telomerase activity in 8 benign, 3 borderline-malignant, 14 malignant tumors, and 6 normal ovarian epithelia were 0.080 ± 0.070 (mean ± SD), 0.408 ± 0.208, 1.659 ± 0.930 and 0.086 ± 0.060, respectively. Additionally, telomerase activity increased significantly with the advance of his-tologic differentiated grade in malignant ovarian tumors. However, no significant relationship was found between telomerase activity and tumor clinical stage and histological types. Conclusions: Our data suggest that the activation of telomerase might play an important role during ovarian carcinogenesis and progression. The increase of telomerase activity is one of the impotrant steps in the infinite proliferation of ovarian carcinoma and might be a useful biomarker for the diagnosis of ovarian carcinoma.