大肠癌胃泌素mRNA表达的临床研究

Clinical Investigation of Expression of Gastrin mRNA in Colorectal Carcinoma

目的 研究胃泌素 (gastrin ,GAS)mRNA在大肠癌癌组织、近旁粘膜 (距癌组织 <3cm)、远旁粘膜 (距癌组织 >8cm)的表达变化及与临床病理参数的关系。方法 收集手术切除的大肠癌新鲜标本 5 0例 ,分别取癌组织、近旁粘膜和远旁粘膜 ,匀浆提取RNA ,逆转录巢式PCR扩增检测GASmRNA表达情况。结果 GASmRNA阳性表达率 :癌组织高于近旁粘膜 (P <0 .0 1) ,近旁粘膜高于远旁粘膜 (P <0 .0 5 ) ;低分化癌较高、中分化癌为高 (P <0 .0 1) ;C、D期较A、B期为高 (P <0 .0 1) ;粘液腺癌、印戒细胞癌、未分化癌较乳头状腺癌、管状腺癌为高 (P <0 .0 5 )。结论 大肠癌细胞或组织自身分泌GAS ,调节大肠癌的生长 ;GAS的表达变化是大肠癌发病原因之一 ;GASmRNA的表达与大肠癌的分化程度、临床分期、病理类型关系密切。

Objective\ To investigate the expression of gastrin (GAS) mRNA in cancer tissues, cancer adjacent mucosa (CAM) and cancer distant mucosa (CDM) of colorectal carcinoma,and its relation to histological type, cell differentiation and clinicopathological stage. Methods\ The fresh specimens were collected from 50 cases of colorectal carcinoma excised, which include cancer tissues, CAM and CDM. After extraction and reverse transcription ,gastrin mRNA were detected by using nested PCR with specific primers. Results\ The percentage of GAS mRNA expression in cancer tissues is higher than that in CAM and CDM , higher in poorly differentiated carcinoma than in well differentiated carcinoma, higher in Dukes C, D stages than in Dukes A, B stages, and higher in mucoid adenocarcimoma, signet ring cell carcinoma and undifferentiated carcinoma than in papillary adenocarcinoma and tubular adenocarcinoma . Conclusion\ Colorectal carcinoma expresses GAS to regulate its growth for itself. The change of GAS expression in colorectal carcinoma is one of the pathogenic causes of colorectal carcinoma . The expression of GAS is related closely to histological type, cell differentiation and clinicopathological stage.