促排卵周期卵泡发育与胰岛素样生长因子Ⅰ及其受体mRNA关系的研究

Investigation on the relationship among follicular development and insulin-like growth factor-Ⅰ and its receptor mRNA during ovulation stimulation period

目的 探讨促排卵周期卵泡发育和体内胰岛素样生长因子Ⅰ (IGF Ⅰ )及IGF Ⅰ受体(IGF ⅠR)mRNA的关系。方法 用ELISA法检测促排卵周期血清及卵泡液IGF Ⅰ水平 ,并分析血清或卵泡液IGF Ⅰ浓度与卵泡发育数关系。用RT PCR法检测颗粒细胞IGF ⅠmRNA表达量。结果 促排卵前、后血清及卵泡液IGF Ⅰ浓度差异无显著性 ;低反应组 (卵泡数≤ 3个 )颗粒细胞IGF ⅠmRNA明显低于中、高反应组 (卵泡数分别为 4～ 13个和≥ 14个 )。结论 血清IGF Ⅰ水平不能反映卵泡发育数 ,颗粒细胞IGF ⅠmRNA的量可能影响促排卵周期卵巢对促排卵药物的反应。

Objective To observe the relationship among follicular development and insulin-like growth factor Ⅰ (IGF-Ⅰ) and IGF-Ⅰ receptor (IGF-ⅠR) mRNA during ovulation stimulation period in an in-vitro fertilization (IVF) program. Methods IGF-Ⅰ levels in serum samples obtained before (menstrual day 3) and after stimulated (at the time of ovum pick-up) and follicular fluid samples were measured by ELISA. The expression of IGF-Ⅰ mRNA in the granulosa cell obtained from follicular aspiration was determined by RT-PCR. Results There was no significant difference between IGF-Ⅰ levels of serum samples before or after stimulated and follicular fluid. In patients with less than 4 follicls (diameter≥14 mm) at the time of ovum pick-up the IGF-Ⅰ mRNA level was significantly lower than that in patients with more than 4 follicles. Conclusion The serum IGF-Ⅰ levels fail to predict the numbers of follicular development; the levels of IGF-ⅠR mRNA in granulosa cells forecast the responsiveness of ovary to the stimulation of gonadotropins in an IVF program.