MDS病人血浆IL-10的检测及临床意义

DETECTION OF PLASMA INTERLEUKIN-10 LEVEL AND ITS CLINICAL SIGNIFICANCE IN PATIENTS WITH MYELODYSPLASTIC SYNDROME

①目的 探讨骨髓增生异常综合征 (MDS)病人血浆白细胞介素 10 (IL 10 )的水平及其临床意义。②方法 用酶联免疫夹心 (ELISA)法 ,检测 2 5例MDS病人及 2 4例正常人血浆中IL 10水平 ,用流式细胞仪检测T淋巴细胞亚群。③结果 MDS难治性贫血 (RA)组病人及MDS难治性贫血伴有原始细胞增多 (RAEB)组病人血浆IL 10水平明显高于正常对照组 ,差异有显著性 (F =10 .94 1,q =2 .36 ,4 .2 6 ,P <0 .0 5 ) ;RAEB病人血浆IL 10水平高于RA组 ,差异有显著性 (q =2 .4 6 ,P <0 .0 5 )。MDS病人IL 10水平与CD+ 3 ,CD+ 8细胞百分率呈正相关 (r =0 .2 5 ,0 .5 6 ,P <0 .0 5 ) ,与CD+ 4 细胞百分率呈负相关 (r =- 0 .4 8,P <0 .0 1)。④结论 检测IL

Objective\ To investigate the level of interleukin 10(IL 10) and its clinical significance in patients with myelodysplastic syndrome (MDS). \ Methods\ Plasma levels of IL 10 in 25 MDS patients and 24 normal controls were assayed by ELISA and the subgroups of T cell were detected. \ Results\ The level of IL 10 was increased significantly in refractory anemia (RA) patients and refractory anemia with excess blasts (RAEB) patients than that in normal controls, the difference was significant( F=10.941,q=2.36, 4.26, P <0.05). The level IL 10 in RAEB group was much higher than that in RA group, the difference was significant ( q=2.46, P <0.05). The IL 10 level was positively correlated with the percentage of CD + 3,CD + 8cells( r= 0.25 ,0.56, P <0.05),and negatively with the percentage of CD + 4cells ( r=-0.48, P <0.01). \ Conclusion\ The detection of IL 10 is helpful for guiding the category of MDS and predicting its prognosis.