摘要
目的 观察褪黑素对不同因素诱导的痴呆动物中枢神经细胞产生NO的影响。方法 分别采用淀粉样 β多肽2 5 35片段 (Aβ2 5 3 5)和D 半乳糖 (D gal)诱导大鼠和小鼠学习记忆障碍模型 ,并采用胎鼠皮质 海马神经细胞培养来观察Aβ2 5 3 5和褪黑素对其产生NO的影响 ;NO的测定采用比色法。结果 在Aβ2 5 3 5诱导的大鼠学习记忆障碍模型上 ,大鼠脑组织匀浆中NO的水平各实验组间差异无显著性 ;小鼠皮下注射D gal 3个月后脑组织匀浆中NO含量较对照组降低 (P <0 0 1) ,褪黑素 (1、10mg/kg)可降低的NO水平 (P <0 0 1) ;Aβ2 5 3 5(1× 10 -7、1× 10 -6、1× 10 -5mol/L)能降低胎鼠皮质 海马细胞培养上清液中NO的含量 ,褪黑素 (1×10 -5mol/L)可对抗这种作用。
Objective To investigate the effect of melatonin on NO level in brain tissue of amnesic animals. Methods The amnesic animal models were induced by amyloid β peptide fragment 25-35 (Aβ 25-35) and D-galactose (D-gal). NO level was determined by spectrophotometry. Results NO level in brain tissue in Aβ 25-35-treated rats did not significantly differ from that in corresponding control group. However, NO level of the brain homogenates in mice treated with D-gal was decreased. In cultured cortical-hippocampal neurons supernatent, NO level was also decreased. Melatonin increased NO level at the doses of 1 and 10 mg/kg (ig×3 mon) in mice brain homogenates treated with D-gal and at the concentration of 1×10 -5 mol/L in cultured cortical-hippocampal neurons supernatent. Conclusion Effect of melaton on NO level of brain depends on local functional state.
出处
《安徽医科大学学报》
CAS
2002年第1期25-28,共4页
Acta Universitatis Medicinalis Anhui
关键词
褪黑激素
学习
记忆
一氧化氮
痴呆
melatonin/pharmacology
learning
memory
nitric oxide
animal
