肾复康胶囊的抗炎作用与NO的关系研究

Experiments on the relationship between Anti-inflammation effects of Shengfukang capsule(SFK) and the effects of SFK on NO level

目的 为探讨肾复康胶囊治疗肾炎的机理 ,研究肾复康的抗炎作用与NO的关系。方法 应用镉还原柱层析和Griess反应法测定NO的稳定代谢产物NO2 - (NO3- )。结果 肾复康对正常大鼠血、尿中亚硝酸盐 (硝酸盐 ) [NO2 - (NO3- ) ]含量无显著影响 ;能不同程度地抑制角叉菜胶所致的大鼠足跖肿胀 ,并显著地降低血清、尿液和炎性足跖组织中NO2 - (NO3- )含量 ;使棉球肉芽肿大鼠模型中棉球肉芽肿重量减轻 ,显著降低血清、尿液和炎症组织中NO2 - (NO3- )的含量。结论 肾复康对正常大鼠体内NO的产生没有影响 ;其抗炎 (大鼠角叉菜胶性足跖肿胀和棉球肉芽肿 )作用与抑制其体内NO水平有关。

Objective The relationship between anti inflammation effects of Shengfukang capsule (SFK) and its effects on NO production in rats was explored. Methods NO 2 -(NO 3 -) levels in serum, urine and inflamed tissues, which are stable end-products of NO, were detected with cadmium reduction column and Griess colorimetry.Results In normal rats, 0.363, 0.726, 1.452 g·kg -1 SFK of had no significant effects on NO levels in both serum and urine ( P >0.05). In rats with carrageenin induced paw oedema, 0.363, 0.726, 1.452 g·kg -1 SFK reduced dose-dependently oedema degree of the inflamed paw ( P <0.01). SFK reduced NO levels in serum, urine and inflamed tissue also( P <0.01). Edema degrees of the inflamed paw in rats reduced by SFK were positively correlative with NO levels in tissue of inflamed paw ( P <0.05). In rats with cotton ballinduced granulama, 0.363, 0 726, 1.452 g·kg -1 SFK could reduce the weight of granulama and NO level in serum, urine and granulation tissue. Conclusion The results suggested that the anti-inflammatory action of SFK was partly due to its inhibition on NO production.