复方半枝莲防治二乙基亚硝胺诱发大鼠肝癌的研究

Prevention and Treatment of Herba Scutellariae Barbatae Compound on Diethylnitrosamine-induced Hepatocarcinoma of Rat

目的 :研究复方半枝莲 (SBC)对二乙基亚硝胺 (DEN)诱发大鼠肝癌的防治作用。方法 :利用免疫组化、流式细胞仪、血清和组织生化等检测方法 ,分别在第 14周和第 2 4周观察 SBC对 DEN诱发的肝癌形成过程的影响。结果 :中药组 14周时大鼠肝脏异型性增生灶明显较模型组少 ,2 4周时形成的肝癌结节小而少 ,模型组、中药组肝癌发生率分别为 75 .0 %、5 0 .0 % ;免疫组化显示中药组大鼠肝组织谷胱甘肽 - S-转移酶胎盘型阳性灶面积明显低于模型组 ;肝组织匀浆上清液谷胱甘肽 - S-转移酶含量以及血清 λ-谷氨酰转移酶、碱性磷酸酶、谷丙转氨酶含量也明显低于模型组 ;流式细胞仪检测结果显示 ,中药组大鼠肝细胞 G0 - G1 期比例下降 ,G2 - M期比例升高。结论 :SBC能抑制癌前病变 ,延缓肝癌的形成 ,降低肝癌发病率 ,其作用机制之一可能为阻滞 G2 - M期细胞进展 。

Objective:To study the prevention and treatment of Herba Scutellariae Barbatae compound (SBC) on diethylnitrosamine (DEN)-induced hepatocarcinoma of rat. Methods: The rats of DEN group received DEN at a dose of 5 mg/kg in drinking water. The rats of SBC group received SBC besides the same dose of DEN. Immunohistochemistry, flow cytometry, serum and tissue biochemistry methods were used to study the effect of SBC on the development of DEN induced hepatocarcinoma of rats at 14th and 24th week respectively.Results:After 14 weeks,the number of heterotypic hyperplastic foci area in the liver of the SBC group was significantly less than that in the DEN group. At 24th week, cancerous nodes in the liver of the SBC group were much smaller and less than those of th control group. The incidence of hepatocarcinoma in the DEN group and SBC group was 75.0 % and 50.0 % respectively. The GSTP positive foci area of the liver tissue in the SBC group was smaller than that in the DEN group. The content of GST in liver homogenate supernatant and serum GGT, AKP and ALT levels in the SBC group were significantly lower than those in the DEN group respectively. The result of flow cytometric analysis showed that comparing with the DEN group, the percentage of G0-G1 phase cells in the SBC group was decreased and the percentage of G2 M phase cells increased. Conclusion:SBC could inhibit preneoplastic lesion, postpone hepatocarcinogenesis and decrease the incidence of hepatocarcinoma. One of the mechanisms might be to postpone advancement of G2 M phase cells and to retard dedifferentiation and malignant proliferation of hepatocytes.