益肝浓缩煎剂抗肝纤维化的实验研究

Experimental Study on Concentrated Yigan Decoction in the Treatment of Liver Fibrosis

目的 :观察益肝浓缩煎剂及其组方中药丹参、黄芪对肝纤维化大鼠肝组织病变的影响 ,并探讨其抗肝纤维化的作用机制。方法 :以皮下注射 4 0 % CCl4 花生油溶液诱导产生大鼠肝纤维化模型 ,并于益肝浓缩煎剂、丹参、黄芪应用治疗前后处死大鼠 ,留取肝组织标本 ,切片并 Masson染色 ,观察胶原纤维增生情况。结果 :益肝浓缩煎剂肝内胶原纤维增生程度明显减轻 ,其疗效优于丹参与黄芪组 :活血化瘀中药有明显抗肝纤维化作用 ;益气健脾中药具有一定的抗肝纤维化作用 ;活血化瘀中药抗肝纤维化作用优于益气健脾中药 ;益肝浓缩煎剂抗肝纤维化作用 ,优于单纯的活血化瘀中药或益气健脾中药。结论 :益肝浓缩煎剂具有良好的抗肝纤维化作用 。

Objective:To study the effect and mechanism of concentrated Yigan decoction (CYD), radix salviae miltiorrhizae (RSM) and radix astragali (RA) in the treatment of liver fibrosis. Methods:The SD rat model of liver fibrosis was induced by subcutaneous injection of 40 % CCl4. After the treatment, the collagen in liver tissue was investigated with Masson staining. Results:The degree of liver fibrosis was obviously alleviated in the CYD group as compared with that in RSM group and RA group. Herb with the function of reinforcing the circulation of blood and removing stasis can obviously decrease the degree of liver fibrosis. Herb with the function of tonifying qi and spleen can decrease the degree of liver fibrosis. The effect of the former is better than that of the latter. The effect of CYD was better than that of RSM and RA. Conclusion:CYD had an affirmative effect in treating liver fibrosis and could obviously decrease the degree of liver fibrosis and improve the pathological change of liver fibrosis in rats.