胃祺Ⅱ号方对大鼠胃窦和胃底一氧化氮含量的影响

Effect of Weiqi Decoction Ⅱ on the Content of Nitric Oxide in the Gastric Antrum and Fundus Tissue of Rats

目的 :观察胃祺 号方对大鼠胃窦、胃底部一氧化氮 (NO)含量的影响 ,探讨其可能的作用机制。方法 :采用大鼠胃电节律失常模型 ,以硝酸还原酶法测定胃窦和胃底部 NO的含量。结果 :与正常组比较 ,模型组大鼠胃窦 NO含量显著升高 (P <0 .0 1) ,而胃底部 NO含量显著降低 (P <0 .0 5 )。胃祺 号方组胃窦部 NO的含量较模型组显著降低 (P <0 .0 1) ,而胃底部 NO的含量较模型组显著升高 (P <0 .0 5 ) ,与正常组相仿。结论 :胃祺 号方能升高胃底部 NO的含量 ,降低胃窦部 NO的含量 ,可能是其治疗功能性消化不良的重要机制。

Objective:To observe the effect of Weiqi decoction Ⅱ on the content of nitric oxide (NO) in the gastric antrum and fundus tissue of rats and investigate the mechanism.Methods:The rat model of electrogastric dysrhythmias was used. The content of NO in the antrum and fundus tissue was measured with the method of nitrate reductase. Results:Compared with that in the normal control group, the content of NO in the model group was obviously increased in the antrum tissue while decreased in the fundus tissue significantly (P<0.01 or 0.05). Compared with that in the model group, the content of NO in the Weiqi decoction Ⅱgroup was reduced in the antrum tissue while enhanced in the fundus tissue significantly (P<0.01 or <0.05), and was similar to that in the normal control group. Conclusion:Weiqi decoction Ⅱ can increase the content of NO in the fundus and decrease the content of NO in the antrum significantly, which may be an important mechanism of treating functional dyspepsia.