血红蛋白值校正溶血胆碱酯酶活力值的研究

STUDY OF THE ADJUSTED CHOLINESTEERASE ACTIVITY OF HEMOLYSED SAMOLES BY HEMOGLOBIN

目的 探讨健康成人溶血样品胆碱酯酶活力 (h -ChE) ,未溶血全血胆碱酯酶活力 (b1 -ChE)值之间的比例关系 ,并用血红蛋白值 (Hb)对h -ChE进行校正。方法 采用国家标准GB7794- 87三氯化铁比色法测定h -ChE及b1 -ChE。结果 h -ChE与b1-ChE之间呈高度正相关 ,r =0 .975 ,P <0 .0 1。Hb值校正h -ChE ,其校正值接近或略高于正常值。结论 用Hb值校正h -ChE可避免离体血样后处理过程中因溶血干扰比色所致测定值误差。缩小男。

Objective To study the relationship between the activity of cholinesterase in the hemolysed samples (h-Che) and that of the whole blood cholinesterase (bl-ChE) in the ordinary adults' samples, and to adjust the h-ChE by hemoglobin (Hb) value. Methods The h-ChE and bl-ChE were measured according to the national standard GB7794-87 tricloiron colorimetric analysis method. Results There is a high positive relevance between the h-ChE and bl-ChE (r=0.975, P<0.0001). The h-ChE adjusted by Hb was close to or slightly higher than the normal value. Conclusion Using Hb to adjust the h-ChE can avoid the measuring errors due to the influence of the hemolysis which occurs in the aftertreatment of the blood samples, and can also reduce the scattering degree of the measured values between males and females or among different populations.