2D—NMR对皂皮酸—3—氧—葡萄糖醛酸^(13)C,~1H的完全指定——介绍HMBC和HOHAHA

COMPLETE ^(13)C, ~1H ASSIGNMENTS FOR QUILLAIC ACID-3-O-GLUCURONIC ACID BY TWO-DIMENSIONAL NMR EXPERIMENTS -Introduction of HMBC and HOHAHA

HMBC是一种测定远程偶合~1H—^(13)C相关的十分灵敏的方法,特别适用于检测和甲基质子远程偶合(~2J,~3J)的碳.HOHAHA谱显示出多次Relay信息,选择适当参数可通过一次实验得到独立自旋体系中所有质子相关信息.本文用HMBC和HOHAHA实验结合同核(~1H)COSY和导核(^(13)C-~1H)COSY确认了Qwhaic acid—3—O—glucuronic acid分子中所有^(13)C和~1H的归属.

HMBC is a sensitive method for determining long-range 1H - 13C connectivity. It is suitable for conelating methyl protons with carbon atoms through two-and three - bond, especially. HOHAHA provides relay information over all protons in spin coupling network. In this paper HMBC and HOHAHA, in combination with 1H COSY and 13C-1H COSY have been used to unambiguously assign 13C and 1H resonances for Quillaic Acid-3-O -giucuronic acid.