猪血浆α_1—抗胰蛋白酶的紫外光谱和园二色谱

THE U. V. ABSORBANCE SPECTRUM AND C. D. SPECTRUM OF PORCINE PLASMA α_1-ANTITRYPSIN

本文报告了对猪血浆α_1抗胰蛋白酶的紫外吸收光谱和园二色谱的测定结果。从紫外光谱分析得知猪α_1抗胰蛋白酶表现出典型的蛋白质吸收特征,其摩尔消光系数E_(280nm)~1％1cm 等于5.25;从(?)二色谱分析得知该蛋白质分子中合有约8％的α螺旋,50％的β-折叠,其余均为无规则卷曲构象。本测定结果对猪α_1-抗胰蛋白酶的性质、结构和功能的研究打下了基础。

The paper reported a determination about the U. V. absorbance spectrum and C. D. spectrum of porcine plasma α_1—antitrypsin. This proteinase inhibitor had a typical protein U. V. absorbance properity and E_(280nm)^(1%1cm) 5.25, contained 8%α-helix, 50%β-pleated sheet and the rest aperiodic structure obtained from C. D. spectrum. These results laid a foundation for researching the properties, structures and functions of porcine plasma α_1-antitrypsin.