rhIL-15和rhIL-2诱生的LAK细胞特性比较

Functional and phenotypic comparison of lymphokine-activated killer cells induced by rhIL-15 and rhIL-2

目的：研究ｒｈＩＬ－１５激活的ＬＡＫ细胞的增殖、细胞毒作用及相关表型的变化。方法：通过用不同浓度的ｒｈＩＬ－１５和ｒｈＩＬ－２分别诱生 ＬＡＫ细胞，研究二者诱生的 ＬＡＫ细胞的增殖状况；当 ｒｈＩＬ－２和 ｒｈＩＬ－１５为 １５００ Ｕ／ｍｌ时，ＭＴＴ法检测 ＬＡＫ细胞的细胞毒作用，利用流式细胞仪检测细胞表面ＣＤ分子表达情况。结果：ＬＡＫ细胞的增殖对ｒｈＩＬ－２和ｒｈＩＬ－１５均具有时间和剂量依赖性，且无明显差异；终浓度为 １５００ Ｕ／ｍｌ时，ｒｈＩＬ－１５诱生的 ＬＡＫ细胞杀伤 Ｋ５６２细胞的能力强于 ｒｈＩＬ－２诱生的 ＬＡＫ细胞，而对Ｒａｊｉ细胞的杀伤活性，ｒｈＩＬ－２诱生的ＬＡＫ细胞却明显高于 ｒｈＩＬ－１５诱生的 ＩＡＫ细胞，且二者杀伤活性均具有时间依赖性；同时ｒｈＩＬ－１５诱生的ＬＡＫ细胞的ＣＤ３－ＣＤ５６＋细胞、Ｃｄ９４＋细胞百分率均高于ｒｈＩＬ－２诱生的ＬＡＫ细胞。结论：ｒｈＩＬ－１５在体内对ＮＫ细胞功能可能具有较强的调节作用。

Objective: To study the role of IL-15 on the proliferation, cytotoxicity and phenotype of PBMCs. Methods: PBMCs were acquired from peripheral blood and cultured with different dose recombinant human IL-2( rhIL-2) and recombinant human IL-15 ( rhIL-15) The total number of cells were counted. The lyric activity of LAK cells against LAK-sensitive cell line(Raji) and NK-sensitive cell line(K562) was determined using MTT calorimetric assay. The flow cytometer was used to compare and analyse the lymphocytes phenotypes. Results: The effect of rhIL-15 on PBMCs proliferation was both time-and dose-dependent, and the effect of rhlL-2 on proliferation was not significantly different with rhIL-15 The NK activity against K562 induced by rhIL-15 was higher than that of rhIL-2,but LAK activity against Raji was lower than that of rhIL-2.The percentage of CD3- CD56+ and CD94+ cell in LAK cells induced by rhIL-15 are all higher than that of rhIL-2.Condusion:IL-15 is even more effective on NK cells than IL-2.