正常人外周血和胸腺T细胞中TCR DδX基因重排的特点

The feature of TCR DδX gene rearrangements in peripheral blood T cells and thymocytes from healthy individual

目的：分析正常人外周血和胸腺细胞中 ＴＣＲ ＤδＸ基因重排分布特点。方法：利用半巢式 ＰＣＲ扩增 １０例正常人外周血单个核细胞（ＰＢＭＣｓ）、４例分选的外周血 ＣＤ３＋Ｔ细胞和 ７例正常人胸腺细胞 ＤＮＡ的 ＴＣＲ ＤδＸ基因与ＪδＸ、Ｄδ３和 Ｊａ重排的情况，从不同含量 ＤＮＡ的 ＰＣＲ分析，了解其重排分布频率。结果：ＴＣＲ ＤδＸ分别与 ＪδＸ、Ｄδ３和 Ｊａ的重排均可见于多数外周血Ｔ细胞和胸腺细胞中，对不同含量的ＤＮＡ的ＰＣＲ分析显示ＴＣＲ ＤδＸ重排的分布频率在外周血和胸腺细胞有所不同。结论：ＴＣＲ ＤδＸ－ Ｊａ的重排最常见于成熟和未成熟Ｔ细胞中，而ＴＣＲ ＤδＸ－Ｄδ３则在未成熟Ｔ细胞中多见。

Objective: To investigate the distribution feature of TCR D^X gene rearrangement in peripheral blood T cells and thymocytes from healthy individuals. Methods: The rearrangement segments of TCR DδX gene with JδX, Dδ3 or Ja were amplified in genomic DNA from peripheral blood mononuclear cells(PBMCs) of 10 cases, sorted CD3 + T cells from peripheral blood of 4 cases and thymocytes from 7 cases, by using semi-nested PCR. Different amounts of DNA from all samples were amplified to estimate the frequency of DδX gene rearrangements. Results: The rearrangements of TCR DδX with JδX,Dδ3 or PJa respectively could be found in the most samples of peripheral blood or thymocytes.The frequency of DδX rearrangements were different in peripheral blood cells and thymocytes by analysis of PCR with different amounts DNA. Conclusion: The TCR DδX, Ja was the most frequent rearrangement in mature and immature T cells, whereas TCR DδX,Dδ3 were more frequently rearranged in immature T cells.