HPLC法测定诺氟沙星胶囊含量的改进

Improvement of determination of norfloxacin capsules by HPLC

目的 :改进诺氟沙星胶囊含量HPLC测定方法。方法 :采用反相高效液相色谱法。色谱柱 :SpherisorbC18柱 ,流动相 :磷酸 -三乙胺缓冲液水乙腈 (6 0 30 10 ) ,流速 :1 0ml·min- 1,检测波长 :2 78nm。结果 :诺氟沙星的浓度在 4 8～ 96 μg·ml- 1范围内线性关系良好 ,回归方程 :A =2 19× 10 4 + 7 4 0×10 4 C(r =0 99998) ,加样回收率平均值为 98 5 %。RSD =0 5 % ,n =5 )。结论 :用本方法测定诺氟沙星胶囊的含量快速、重现性好、专属性强。

OBJECTIVE:Norfloxacin capsules was determined by a improved HPLC method. METHODS:The separation was performed on a spherisorb C 18 column.The mobile phase was composed of phosphoric acid-triethylamine buffer-water-acetonitrile(603010).The eluate with a flow rate of 1.0ml·min -1 was detected at the wavelength of 278nm. RESULTS:The calibration curves were good linear within the range of 4.8～96μg·ml -1 ( r =0.99998).The average recovery of norfloxacin was 98.5%,with RSD of 0 5%( n =5). CONCLUSION:The improved method is rapid,good repeatabitity,accurate,reliable and suitable for determination of norfloxacin capsules.