HPLC法测定盐酸氯胺酮控释片中盐酸氯胺酮的含量

Determination of the content of sustained-release ketamine hydrochloride tablets by high-performance liquid chromatography

目的 :建立盐酸氯胺酮控释片中盐酸氯胺酮的含量测定方法。方法 :采用高效液相色谱法 ,以C18为固定相 ,乙腈 -水 (30 70 )为流动相 ,检测波长为 2 6 9nm。结果 :盐酸氯胺酮在 31～ 310 μg·ml- 1范围内 ,其峰面积值与浓度具有良好的线性关系 (r =0 9991) ,该方法平均回收率为 97 4 %。RSD为 0 6 6 % (n =5 )。结论 :该方法简便易行 ,精密度高。

OBJECTIVE:To establish a method of the content of sustained release ketamine hydrochloride tablets METHODS:HPLC method was used with C18 column as stationary phase and acetonitrile-water(7030)as mobile phase The detection wavelength was 269nm RESULTS:The calibration curve is linear in the range of 31～310μg·ml -1 for ketamine hydrochloride The average recorery was 97 4% with RSD of 0 66% ( n =5) CONCLUSION:The method is simple,reliable,accurate and can be used for the determination of the content of sustained-release ketamine hydrochloride tablets