血管内皮细胞生长因子在结直肠组织中的表达及其意义

Expression of vascular endothelial growth factor in colorectal cancer and its clinical significance

目的 研究血管内皮细胞生长因子 (VEGF)和结直肠癌的关系。方法 应用流式细胞技术 (FCM)对结直肠癌肿瘤组织和癌旁正常粘膜组织标本进行VEGF的测定 ,结果用阳性细胞百分率和平均荧光强度表示。结合临床资料探讨VEGF和结直肠癌的生物学行为以及发生、发展与预后的关系。结果 VEGF在结直肠癌肿瘤组织中阳性细胞表达百分率为 5 7%± 2 9%、阳性细胞平均荧光强度为 2 4 %± 11% ,在癌旁正常粘膜组织标本中的阳性细胞表达百分率为 4 2 %± 2 4 %、阳性细胞平均荧光强度为 16 %± 7% ,两者比较差异具有显著意义 (P <0 0 5 ) ,且VEGF的阳性表达和结直肠癌Dukes分期相关 (P <0 0 1)。结论 血管内皮细胞生长因子 (VEGF)与结直肠癌的发生、发展和预后相关 ,但与结直肠癌生物学分化程度之间的关系还有待进一步验证。

Objective To evaluate the relationship between vascular endothelial growth factor (VEGF) and colorectal cancer. Methods Samples of cancer and adjacent normal mucosa were taken from 34 patients with colorectal cancer. The percentage and average fluorescence intensity pf VEGF positive cells in these samples were examined by using flow cytometry. Results The percentage of VEGF positive cells was 57%±29% in cancer tissue and 42%±24% in normal tissue ( P <0.05). The average fluorescence intensity of VEGF positive cells was 24%±11% in cancer tissue and 16%±7% in normal tissue ( P <0.01). The percentage of VEGF positive cells was 30%±22% in cancer of Dukes stage B and 72%±18% in cancer of Dukes stage C ( P <0.01). The average fluorescence intensity was 18%±25% in cancer of Dukes stage B and 27%±12% in cancer of Dukes stage C ( P <0.01). Conclusion VEGF is associated with the development and prognosis of colorectal cancer. Its relation with degree of differentiation of colorectal cancer remains to be studied.