糖酐酯对高血糖大鼠溶栓治疗的影响

Effects of thrombolytce therapy combined with inhibition of leukocytes infiltration in a hyperglycemic rat embolic stroke model

目的探讨高血糖与溶栓治疗后白细胞浸润的关系 ,以及糖酐酯对高血糖大鼠溶栓治疗的影响。方法通过复制高血糖大鼠模型 ,自体血栓栓塞大脑中动脉 ,缺血 30分钟静脉予以糖酐酯或生理盐水 ,缺血 2小时经颈外动脉注入尿激酶溶栓 ,观察缺血 2 4小时浸润白细胞数及梗死灶大小。结果缺血 2 4小时 ,高血糖大鼠溶栓组与正常血糖大鼠溶栓组比较 ,缺血周边区浸润白细胞数明显增多 (P <0 0 1) ,梗死灶增大 (P <0 0 5 )。高血糖大鼠联合糖酐酯溶栓组较高血糖大鼠溶栓组浸润白细胞数减少 (P <0 0 1) ,梗死灶减小 (P <0 0 5 ) ;与正常血糖大鼠溶栓组比较 ,白细胞数减少 (P <0 0 5 ) ,梗死灶虽有减小的趋势 ,但差异无显著意义。结论高血糖通过增加白细胞浸润加重溶栓治疗后脑组织损伤 ;糖酐酯可以抑制高血糖大鼠溶栓治疗后白细胞的浸润 ,减小梗死灶。

Objective To evaluate the relationship between hyperglycemia and leukocytes infiltration and the effects of dextran sulfate on thrombolytic therapy in the hyperglycemic rat embolic stroke.Methods Dextran sulfate or saline was intravenously administered after 0.5h ischemia in thromboembolic model of hyperglycemic rat,which produced by intraperitioneally injection of 50% dextrose 30 minutes before ischemia.All rats were received urokinase injection after 2h ours ischemia.leukocytes infiltration and infarct size were evaluated at 24h ours after ischemia.Results Treated with urokinase alone,the number of leukocytes infiltration and infarct size in hyperglycemic rat were increased compared with those in normoglycemic rat ( P <0 01, P <0 05).In hyperglycemic rats,treated with combination of urokinase and dextran sulfate,number of leukocytes infiltration and infarct size were decreased compared with those in treated with urokinase alone ( P <0 01, P <0 05).Conclusion Hyperglycemia could exacerbate ischemic brain injury after thrombolysis through leukocytes infiltration.Combination of dextran sulfate and urokinase could inhibit leukocytes infiltration and reduce infarct volume in hyperglycemic rat.