启东肝癌组织p53过表达的免疫组化检测

The p53 overexpression in Qidong liver cancer tissues analysed immunohistochemically

目的 了解p5 3突变在启东地区的肝癌发生中所起的作用。方法 应用免疫组化 (IHC)的方法对 90例肝癌和 75例癌旁组织切片进行p5 3蛋白检测。结果 在 90例肝癌中 ,有 4 0例 (44 .4 % )在核中表达为强阳性 ,2 5例 (2 7.8% )在核中表达为弱阳性 ;在 75例癌旁中 ,没有细胞核强阳性表达 ,仅 1例 (1.3% )有核弱阳性表达 ;肝癌的p5 3核表达显著高于癌旁 (P =0 .0 0 0 )。另外 ,在肝癌中有 9例 (10 % )为细胞质p5 3表达阳性 ,在癌旁中有 17例 (2 2 .7% )为细胞质表达阳性 ,肝癌的胞质p5 3表达显著低于癌旁 (P =0 .0 2 6 )。结论 p5 3基因突变和p5 3的功能失活在启东的肝癌发展中有着重要意义。

Objective To explore the role of p53 mutation in the development of hepatocellular carcinoma (HCC) in Qidong area, which is one of the high incidence areas. Methods Immunohistochemical method (IHC) was used to detect p53 protein overexpression in 90 HCC tissues and 75 HCC-adjacent non-tumor liver tissues (as the control). Results In the 90 HCCs, 40(44.4%) were strongly positive and 25(27.8%) faintly positive for p53 in nucleus, while in the 75 control tissues, none was strongly positive and only 1( 1.3%) was faintly positive. The HCC's was significantly higher than the control's ( P =0.000). On the other hand, 9(10%) HCCs were positive for p53 in the cytoplasm, but 17(22.7%) control tissues were positive, the HCC's was significantly lower than the control's ( P =0.026). Conclusion p53 mutation and functionally inactivation played an important role in the development of HCC in Qidong area.