先天性肾积水转化生长因子转录水平的研究

Expression of TGF-β_1 in children with congenital hydronephrosis

目的 检测先天性肾积水患肾转化生长因子 β1(TGF β1)mRNA表达的改变 ,探讨TGF β1在小儿先天性肾积水肾损伤中的作用及其临床意义。方法 采用RT PCR技术检测 15例先天性肾积水患儿患肾实质组织、肾盂组织、肾盂输尿管狭窄段组织TGF β1mRNA的表达 ,经凝胶分析系统半定量 ,和 10例正常肾、肾盂及输尿管对照。结果 患肾组织、肾盂及肾盂输尿管狭窄段中TGF β1mRNA的表达增高。结论 先天性肾积水患儿患肾组织TGF β1表达增高 ,TGF

Objective To assess the expression of TGF β 1 mRNA and the role of TGF β 1 in children with congenital hydronephrosis.Methods The expression of TGF β 1 mRNA in renal parenchyma, renal pelvis and pelvic ureteral junction of 15 cases with congenital hydronephrosis was studied using the RT PCR method. With the assistance of gene analysis, the results were campared with those of 10 normal controls.Results The expression of TGF β 1 increased in the affected renal parenchyma, renal pelvis and pelviureteric junction. The difference was statistically significant.Conclusions The expression of TGF β 1 is increased in congenital hydronephrosis. The increase of TGF β 1 expression may be related to chronic renal damage and renal interstitial fibrosis caused by hydronephrosis.