骺阻滞诱导脊柱侧弯动物模型的实验研究

Scoliosis induced by unilateral epiphysiodesis in canine pups

目的 利用动物模型反向证明骺组织生长潜能治疗先天性脊柱侧弯的可能性。方法 对 16只离乳的幼犬分实验组和两个对照组 ,行脊柱一侧的前后方骺切除及骨融合术 ,经过喂养 14周后 ,观察脊柱能否发生侧弯、侧弯程度以及畸形与年龄的关系。结果 16只动物中 13只动物达到预期效果 ,实验组动物脊柱畸形明显 ,并随时间进行性加重。第 14周时 ,实验组动物的平均Cobb角为37.0°± 6 .4 8° ;实验组与两个同期的对照组相比差异有显著性意义 (P1=0 .0 0 0 4 9,P2 =0 .0 0 0 2 7)。结论 幼犬实施前后路联合一侧骺切除和局部融合术可以造成脊柱侧弯。

Objective To introduce a simple and reproducible canine model scoliosis as a research tool for congenital scoliosis.Methods Sixteen 70 day old pups with a body weight of 2,500g-3,500g were randomly divided into three groups: A ( n =6), B ( n =5) and C ( n =5). All pups underwent surgery with the same anesthesia. In group A, combined anterior and posterior unilateral fusion and hemiepiphysiodesis in three successive segments were performed. In group B vertebral body was incised unilaterally without hemiepiphysiodesis. In Group C the same operation as group A was performed on 160 days old pups. Subjects were followed up for 14 weeks after the operations. Experimental results were assessed with histology, radiography and CT examination.Results The spine deformity of group A was more evident than the other groups. Radiological examinations revealed progressive curved deformity of the spine. The Cobb's angle was 27.00° ± 6.98° at 8 th week and 37.00° ± 6.48° at 14 th week. Statistical analyses showed a significant difference between group A and B (P1= 0.00049 ), and group A and C (P2= 0.00027 ).Conclusions In canines, epiphysiodesis induced scoliosis is due to the asymmetric growth of the vertebral bodies. The combined anterior and posterior unilateral fusion and hemiepiphysiodesis surgery in canine pups is feasible.The experiment indicates that correction at an earlier stage is feasible in the management of congenital scoliosis in children.