唾液酸在宫颈癌组织中的表达及意义

Expression of sialic acid in carcinoma of uterine cervix

目的 探讨唾液酸在宫颈癌组织的表达及意义。方法 采用凝集素组织化学染色技术检测了70例宫颈癌组织、3 0例不典型增生组织和 1 0例正常宫颈组织唾液酸的表达 ,应用图像分析系统定量分析其表达水平。结果 唾液酸在非癌细胞中无表达 ,在宫颈癌组织中的表达与病理类型无关 (P >0 .0 5 ) ,在细胞分化程度低、临床分期晚、发生转移、复发及预后差的患者中高表达 (P <0 .0 1或P <0 .0 5 )。

Objective To study the expression of sialic acid in carcinoma of uterine cervix and its relationship with the clinical features. Methods We used biotinylated Jacalin, a special binding lectin of sialic acid, to stain sialic acid in cervical cancer cells by means of lectin\|histochemical technique. The expression of sialic acid was investigated using image analysis system objectively and quantitatively. Results The expression of sialic acid in non\|carcinoma cells could not be detected, the staining intensity indexes of biotinylated Jacalin cervical cancer cells were high significantly in the patients with metastasis, poor cell differentiation, advanced stages ( P <0.01,respectively), or poor prognosis( P <0.05),but were not related to the pathological type( P >0.05). Conclusion The expression of sialic acid in human cervical carcinoma could be used as a marker of malignancy potential as well as a marker of poor prognosis.