p53与HPV16、18型感染在肺鳞癌中的相关研究

Relationship of human papillomavirus types 16 and 18 infection and p53 gene mutation in lung squamous cell carcinoma

目的 探讨HPV与p5 3基因突变在肺鳞癌发病中的相互作用。方法 肺癌组织标本的p5 3基因序列 5～ 8外显子突变分析应用单链构象多态性分析多聚酶链反应EB染色法 (PCR SSCP EB) ,HPV1 6、1 8型DNA相关序列的检测应用PCR法。结果 40例肺鳞癌组织标本p5 3基因突变率为 67.5 % ,其中外显子 5～ 8突变率分别为 1 7.5 %、1 2 .5 %、1 7.5 %、2 0 %。肺鳞癌组织中HPV1 6、1 8DNA相关序列的检出率为 42 .5 %。p5 3基因突变与HPVDNA序列的检出之间呈显著负相关。 结论 p5 3基因突变与HPV感染在肺鳞癌的发病过程中可能存在相互促进作用。

Objective To study the relationship of human papillomavirus (HPV) and p53 gene mutation in the pathogenesis of lung squamous cell carcinoma.Methods PCR\|SSCP\|EB method was used to detected the mutation of exons 5 to 8 of p53 gene,and the HPV types 16,18 DNA related sequences were detected by PCR.Results 67.5% of 40 tumor tissue specimens had p53 gene mutations. The incidences of mutation of exons 5 to 8 were 17.5%,12.5%,17.5% and 20%,respectively. 42.5% of lung squamous cell carcinoma samples were shown to be positive for HPV types 16,18 DNA related squences. There was an inverse correlation between the presence of HPV 16 and 18 infection and mutations of p53 gene.Conclusion The mutation of p53 gene and HPV infections may promote each other in the generation of lung squamous cell carcinoma.