产H_2S志贺Ⅰ型菌的发现与研究

A study on discovery of I type of shigella of Producing H_2S

[目的]鉴定1998年6月自腹泻病人标本中分离的产H2S细菌的种属、血清群、型及其临床价值和流行病学意义。[方法]腹泻病人粪便标本经增菌、分离培养后、挑取可疑菌纯培养,进行生化反应、血清学鉴定。豚鼠角膜试验、鲎试验分别检测未知菌的侵袭力和内毒素,“K-B法”测定细菌对抗生素的敏感性。[结果]该菌为革兰阴性杆状菌,对营养要求不高,在普通培养基上即能生长繁殖,生化学性状符合志贺菌属定义,豚鼠角膜试验显示其有侵袭力,鲎试验表明能产生强烈内毒素,药敏试验证实对多种抗菌素敏感。[结论]经形态学、培养特性、生化学和血清学等微生物学性状的检测,经安徽省卫生防疫站专家的进一步鉴定,确认并命名未知菌为产H2S志贺I型菌,也是引起该腹泻病人腹泻的唯一病原菌。

[Objective]To identify the species, serum type, clinical value and epidemiological significance of bacillus producing H2S, which was seperated from diarrhea patient samples in June, 1998. [Methods] After feces samples of diarrhea were enriched and cultured, we took out suspicious bacterium to culture purely, detect invasiveness and endotaxis by biochemical reaction serum identification, guinea pig conerntest. The bacterial sensitivity to centibiotil was determined by K-B. [Results]The bacterium belongs to Gram negative bacillus, which may grow and reproduct is common culture medium. Its biochemical properly accorded with shigella definition. Guinea pig cornea test showed it had invasivenes and clinical test showed it may roduce strong endotoxin Drug, sensivity test proved it was sensitive to varied antibotics. [Conclusion]The bacterium was determined in morphology, cultural characteristies, biochemistry and serol-ogy and soon, which is called I type shigella producing H2S by experts of Anhui Province Health and Anti - epidemic Station. It was only pathogenic bacteria which induced the patient diaerrhea.