GC/MS法同时检测尿中N,N-二甲基甲酰胺及其代谢产物N-甲基甲酰胺

Simultaneous Determination of N, N - Dimethylformamide and N - Methylformamide, a metabolites of N, N - Dimethylformamide, in human urine by Gas Chromatograhy - Mass Spectrometry

尿样中的N,N-二甲基甲酰胺（DMF）及其代谢产物N-甲基甲酰胺（NMF）用醋酸乙酯萃取、浓缩后,用气相色谱-质谱联用仪分析,全扫描模式（scan）进行定性分析,确定组分出峰时间和特征质量离子,然后选取30.0、59.0、440、73.0m/z进行选择离子扫描（SIM）,作定量分析。DMF和NMF的线性范围分别为0.2～48.0mg/L和0.5～50.0mg/L;相关系数分别为0.998和0.999;最低检出限分别为0.05mg/L和0.15mg/L;相对标准偏差分别为4.0％～6.4％和5.1％～8.4％;样品加标回收率分别为91.1％～97.0％和88.8％～96.3％;样品在4℃的冰箱内至少可保存2周。方法准确性好。灵敏度高、干扰少。是检测尿样中DMF和NMF的较好方法。

A gas chromatogrphy - mass spectrometry method (GC - MS) was developed for detecting N, N - Dimethylformamide and N - Methylformamide in human urine. Qualilative analysis using full scan mode, and quantitative analysis using seleted ion monitoring mode. were employed. The linear range of N, N - Dimethylformamide and N - Methylformamide in the method is 0.2 - 48mg/L and 0. 5 - 50mg/L, the correlation coefficent is 0.998 and 0.999, the detection limit is 0.05mg/L and 0.15mg/L, relative standard deviation is4.0-6.45 and 5.1% - 8.4%,the recoery is 91.1%- 97.0% and 88.8% - 96.3%. Sample can be saved in 4℃ at least 2 week. This method have good correctness, high sensitivity and low interference. It is a good method to determine N, N - Dimethylformamide and N - Methylformamide in human urine.