摘要
目的：探讨β－榄香烯对大鼠胃粘膜癌变发生、发展过程中ｂｃｌ－２基因ｍＲＮＡ表达及端粒酶活性的影响。方法：采用甲基硝基亚硝基肌（ＭＮＮＧ）、１０％ＮａＣｌ混合液加４０％乙醇灌胃建立大鼠胃粘膜癌变模型，设立正常组、模型组及榄香烯组，榄香烯组在造模过程中采用β－榄香烯干预。３０周后，分别采用原位杂交及端粒微孔板杂交法检测大鼠胃粘膜ｂｃｌ－２基因ｍＲＮＡ表达及端粒酶活性。结果：榄香烯组癌变发生率低于模型组。在大鼠胃粘膜癌变的发展过程中，ｂｃｌ－２基因ｍＲＮＡ表达与端粒酶活性呈递增趋势，且两者具高度相关性。结论：下凋ｂｃｌ－２基因表达抑制端粒酶活性，是β－榄香烯重要抗癌机制之一。
Background/Aims: To study the effects of J3-elemene on bcl-2 mRNA gene expression and telomerase activity of gastic cancer in Wistar rats. Methods: We studied the expression of bcl-2 gene mRNA and telomerase activity using in situ hybridization in rats of three groups: control group, model group and elemene group. In the model group gastric cancer was induced by MNNG, 10% sodium chloride, and 40% ethanol. In the elemene group gastric cancer was induced by MNNG and elemene was given at the same time. Results: The occurrence rate of gastric cancer in the elemene group was lower than that of the model group. Along with the development of gastric cancer, the expression of bcl-2 gene mRNA and telomerase activity in gastric mucosa was highly correlated and had a trend to increase. Conclusions: β-elemene can reduce the expression of bcl-2 gene mRNA and inhibit telomerase activity in gastric cancer.
出处
《胃肠病学》
2002年第2期83-85,共3页
Chinese Journal of Gastroenterology
