鸡螺杆菌诱导胃上皮细胞白细胞介素8转录及病理意义

The Transcription of Interleukin-8 Induced by Helicobacter pullorum in Gastric Epithelial Cells and its Pathological Significance

目的：观察鸡螺杆菌（Ｕ．ｐｕｌｌｏｒｕｍ）诱导胃上皮细胞白细胞介素（ＩＬ）８转录并探讨其免疫致病机制。方法：分离 培养Ｈ．ｐｕｌｌｏｒｕｍ菌株及构建带有ＩＬ－８报告基因的人胃癌细胞系Ｌ５Ｆ１１，用液体闪烁计数仪测定荧光素酶（ＩＬ－８转 录）活性。结果：所有１０株Ｈ ．ｐｕｌｌｏｒｕｍ诱导荧光素酶活性较幽门螺杆菌（Ｈ．ｐｙｌｏｎ）野生型ｃａｇ致病岛阴性菌株Ｇ５０ 明显增强（Ｐ＜０．０５～０．００１）。结论：Ｈ ｐｕｌｌｏｒｕｍ通过诱导上皮细胞ＩＬ－８转录而参与其免疫致病过程。

Aims: To observe the ability to induce the transcription of interleukin-8(IL-8) in gastric epithelial cells by Helicobacter pullorum(H. pullorum) and to investigate its immunopathological mechanisms. Methods: H. pu/forum strains were isolated and cocultured with the constructed L5F11 cells and the transcription of IL-8 was indicated by luciferase activity. Resutls: The luciferase activity induced by 10 H. pu/forums strains was much higher than that by H. pylon(cag PAl negative) G50(P<0.05-0.001). Conclusions: The results showed that the immunological pathogenesis of H. pu/forum is likely to be associated with the increase of the transcription of IL-8 in epithelial cells.