摘要
目的 探讨乙酰胆蒽 ( 2 0 MC)诱导小鼠肺肿瘤发生、发展过程中cyclinD1 和p5 3基因表达特点及其意义。方法 小鼠腹腔内注射 2 0 MC ,在不同时间段处死小鼠 ,得到肺部病变共 2 92个 ,其中上皮增生(HP) 31个 ,肺泡细胞腺瘤 (AA) 14 5个 ,乳头状腺瘤 (PA) 6 1个 ,乳头状腺癌 (PC) 5 5个及其复合性病变。应用免疫组化法检测各型病变中cyclinD1 、p5 3基因蛋白表达情况。结果 cyclinD1 癌基因蛋白在HP、AA、PA及PC中均有表达 ,分别为 86 % ( 6 /7)、87% ( 89/10 2 )、73% ( 4 1/5 6 )及 6 8% ( 17/2 5 ) ,cyclinD1 癌基因蛋白在良性病变的表达强于恶性病变 ( χ2 =5 .5 31,P =0 .0 33)。p5 3抑癌基因蛋白表达于腺癌组织、有明显异型性的细胞、肿瘤进展较晚期 ,PC、PA和AA阳性率分别为 40 % ( 16 /40 )、9% ( 6 /6 9)和 3% ( 4 /12 1) ,且分布方式不同 ,HP无表达。癌组织中p5 3基因表达明显强于各良性病变 ( χ2 =41.77,P =0 .0 0 0 ) ,各型病变中以PC的cyclinD1 、p5 3基因均阳性的比率较高 ,为 2 6 % ,HP和AA中两基因无共同表达。结论 cyclinD1 基因可能参与调控肺肿瘤发生过程 ,而p5 3抑癌基因突变则与细胞增恶、肿瘤进展等有密切关系。
Objective To study the correlation of cyclin D-1 oncoprotein with a mutated p53 in pulmonary tumors progression. Methods Thirty-one male A/J strain mice were treated intraperitoneally with a single injection of 20-methylcholanthrene and sacirificed at various ages. A total of 292 lesions in various histological type were obtained. Immunohistochemical methods were employed to detect the expression of cyclin D-1 and p53 in paraffin-embeded tissues. Results Expression of cyclin D-1 oncoprotein was seen in 6 out of 7 (86%) in hyperplasias, 89 out of 102 (87%) in alveolar adenomas, 41 out of 56 (73%) in papillary adenomas, and 17 of 25 (68%) in papillary carcinomas. Cyclin D-1 expression did not correlate with the progression of the lung neoplasms, and tended to decrease in papillary carcinomas than in papillary adenomas, in alveolar adenomas and hyperplasias (χ 2=5.531, P=0.033). Expressions of p53 gene were obviously stronger in malignant lesions than in benign lesions (χ 2=41.77, P=0.000). The p53 showed positive staining in carcinoma and severe atypical cell population. Expression of p53 was seen in 16 out of 40 (40%) in papillary carcinomas, 6 out of 69 (9%) in papillary adenomas, and 4 out of 121 (3%) in alveolar adenoma. No positive p53 stain was observed in hyperplasias. Co-expressive rate of cyclin D-1 and p53 was highest in papillary carcinomas (26%), but did not show in alveolar adenomas and hyperplasias. Conclusion Cyclin D-1 oncoprotein may play an important role in the oncogenesis of lung neoplasms, especially in the early stage. p53 expression increases significantly as the histological grade advances, and may correlate with the progression of pulmonary tumors and the malignant potential of tumor.
出处
《中国肺癌杂志》
CAS
2002年第2期107-111,共5页
Chinese Journal of Lung Cancer
基金
深圳市卫生科技计划重点攻关项目 (1 9980 50 1 1 )资助~~