用于检测猪瘟抗体的免疫金标试纸条的研制与应用研究

Study and apply for detection of pestissuum antibody by colloidal gold strip

以猪瘟抗原包被硝酸纤维素膜的检测区,在其下端附着胶体金标记的猪瘟抗原,组成免疫金标试纸条,根据胶体金免疫层析原理,用该试纸条建立检测猪瘟抗体水平的免疫金标检测法。再用本试纸条检测法与Dot-ELISA及间接血凝法对298份猪、鸡、鸭、鹌鹑、兔、鼠、羊血清进行猪瘟抗体检测,结果符合率达100%。试验结果表明,本法与Dot-ELISA及间接血凝法一样特异、敏感和微量,而且检测时间短、直观,结果容易判定,适用于大面积猪瘟抗体监测普查。

Based on the principle of colloidal g old im-munochromatography,a strip employ ing colloidal gold technique was prepared and applied t o the detection of an-ti -pestissuum antibody.Detection region of the cellulose membrane was coated by pestissuum an tigen.Which is an bad with colloidal gold wsa attached in the area under the detection region.Anti -pestissuum antibody in 298sera sample obtained from pig,chicken,d uck,quail,rabbitand goat was detected by the strip,Dot -ELISA and indirect agglunation method.Result achieved from the methods mentioned above are in complete agre ement.It shows that the method established in this paper bears the same merits as that of the Dot -ELISA and indirect agglunation meth-ods,which is as follows:It can be emp loyed in micro -analysis and observed directly,the result is easy to be jus-tified;it is time saving,and with hi gh speccifity and sensi-tivety.This method is applicable fo r the detection of pestissuum antibody in a wide areas a nd can be applied to the general survey of pestissuum dis ease.