绒山羊随机扩增多态DNA研究

Studies on random amplified polimorphic DNA(RAPD) of cashmere goats

利用RAPD技术对西藏绒山羊、内蒙绒山羊、辽宁绒山羊的遗传变异进行了分析比较 ,用 37个随机引物和 5 5个两两组合的随机引物组合进行筛选 ,筛选出了 5个多态性较为丰富的随机引物 (组合 )。用其对 3个品种 ,每个品种 2 8个个体进行扩增。由RAPD指纹图计算出西藏绒山羊同内蒙绒山羊的遗传距离为 0 0 876 ,西藏绒山羊同辽宁绒山羊的遗传距离为 0 16 0 1,内蒙绒山羊同辽宁绒山羊的遗传距离为 0 0 80 3,西藏绒山羊、内蒙绒山羊、辽宁绒山羊的遗传变异分别为 0 32 6 6 ,0 2 6 2 2 ,0 2 4 75。

Relationship among Tibetan cashmere goats,Inner Mongolia cashmere goats and Liaoning cashmere goats was studied using the technique of random amplified polymorphic DNA(RAPD) One primer and four primer combinations were screened With the five primers and primer combinations,DNA fragments were amplified from the three breeds Each breed has 28 samples According to their RAPD fingerprint maps,the Nei's (1972)standard genetic distance was:0 0876 between Tibetan cashmere goats and Inner Mongolia cashmere goats,0 1601 between Tibetan cashmere goats and Liaoning cashmere goats,0 0803 between the Inner Mongolia cashmere goats and Liaoning cashmere goats,It coincides with their geographic location The genetic variation of Tibetan cashmere goats,Inner Mongolia cashmere goats and Liaoning cashmere goats is 0 3266,0 2622 and 0 2475 respectively It is also consistent with their development history