促进紫杉醇合成真菌诱导物制备方法的选择及组分的分离

Selecting Preparation Methods and Isolating Components of Fungal Elicitor to Enhance Taxol Biosynthesis

Four methods were used to prepare four crude elicitors containing different ingredients from mycelia of fungus F 5: (a) Polysaccharides A containing lipids and proteins; (b) Micromolecular polysaccharides B without lipids and proteins; (c) Macromolecular polysaccharides F without lipids and proteins; (d) Polysaccharides H containing proteins but no lipids. The crude preparation B had the highest ability to induce taxol biosynthesis among other preparations did. The crude preparation B was through Sephadex G15 column to separate two compositions: BⅠ whose molecular weight was over 1500 D and BⅡ whose molecular weight between 700～ 1500 D. The BⅡ activity to induce taxol biosynthesis was 2.3 times higher than that of the crude preparation B, while BⅠ hadn’t the activity to induce taxol biosynthesis. By analysis of PAL activity induced by elicitors, we conclude that the changes of PAL activity could be regarded as a useful physiological indicator to select the effective elicitor.