均匀设计法优化灰树花深层培养基配方

Optimization of submerged culture recipe of Grifola frondosa by uniform design

系统研究了碳源、氮源、无机盐、维生素等培养基因素对灰树花深层发酵菌丝体产量的影响 ,并通过均匀设计法优化了发酵培养基配方。结果表明 :4 6 %玉米粉、3 5 %豆饼粉、0 1%葡萄糖、0 32 %KH2 PO4 、0 0 1%MgSO4 、少量VB1的培养基配方可使菌丝体产量达到最大。采用此配方试验 ,菌丝体产量平均值达 1 77± 0 0 6g/10 0mL。

The effects of a number of factors on mycelia yield of Grifola frondosa fermentation,including carbon source,nitrogen source,inorganic salts,vitamins The recipe of fermentation medium is optimized through uniform design Results show that mycelia yield reaches maximum when adopting a recipe of 4 6% cornpowder,3 5% bean powder,0 1% glucose,0 32%KH 2PO 4 0 01% MgSO 4,small amount of V B1 The average value of mycelial yield reached 1 77±0 06g/100mL with this recipe