水稻耐贮藏基因(lox-3)DH作图群体构建方法的比较研究

Comparison between Two Methods of Establishing DH Population for Mapping lox-3 Gene in Rice (Oryza sativa L.)

以与水稻耐贮藏材料 (DawDam)有丰富分子标记多态性的常规材料 2 0 70、明恢 6 3、Dulur/协青早B为亲本 ,分别与之杂交 ,采用改良一步成苗花药培养法对其F1代进行花药培养 ,以一步成苗法为对照 ,评价新方法在构建耐贮藏基因DH作图群体中的应用潜力。研究结果表明 ,改良一步成苗法显著地提高了愈伤组织分化率、植株再生率、绿苗率、绿苗素质 ,而且得到的加倍单倍体系田间表现良好 ,是构建耐贮藏基因DH作图群体的理想方法 ,但在不同基因型间表现有一定的差异性 ,其中DawDam/ 2 0 70、DawDam/明恢 6 3组合的培养效率有较大增幅。利用改良一步成苗法已获得一定数量的DH群体 。

Crosses were produced by using a storable rice variety (Daw Dam) and 2070,Ming Hui 63,as well as Dulur/Xie Qing Zao B as parents,among these materials there are abundant polymorphisms of DNA molecular markers.Their F 1's were applied to generate DH lines by an improved method of direct induction of pollen plant.The method of the direct induction of pollen plant was used as check treatment to evaluate the improved method.The results showed that the improved method increased the re-differentiation percentage of pollen callus,the percentage of regeneration plantlets,and the percentage of green regeneration plantlets and produced more vigorous seedlings.The improved method was a competent method of establishing DH population for mapping lox-3 gene.It was also showed that the efficiency of the new method was different among genotypes and out of three experimental crosses Daw Dam/2070 and Daw Dam/Minghui 63 showed more efficient.More than 60 DH lines have been obtained in this experiment.