甜瓜不定胚再生植株的染色体数目变异

Variations in Chromosomal Number of Regenerated Melon Plants from Somatic Embryos

本实验以甜瓜“小麦瓜”和“青皮绿肉”品种为试材 ,经不定胚诱导再生植株。分别调查了不同继代培养时间后形成再生植株的染色体数目 ,并将之与对照染色体数目相比较 ,发现通过诱导不定胚所得到的再生植株中存在着一定的变异 ,而且经过不同继代培养时间后 ,所得到的不定胚再生植株的变异程度不同 ,随着时间的增加 ,染色体数目的变异率从 3 3%增加到 30 % ,变异幅度也从 2n =2 3～ 2 4增加到 2n =13～ 48。从而得出结论 :不定胚再生植株染色体数目变异程度随着培养时间的增加而增加 ;培养时间在 1～ 2个月内所得到的不定胚再生植株的变异较少。此外 。

Chromosomal number of different of somatic embryos regenerated plants were investigated in melon variety 'xiaomaigua' and 'Qingpilurou'.Certain variations of chromosomal number were found among the regenerated plants compared with normal sample,and range of variation covered from 2n=23～24 to 2n=13～48 with the increase of generation,the rate from 3 3% to 30%.The results indicated that degree of variation in chromosomal number of somatic embryos regenerated melon plants increased with the time of culture,and those cultured in one to two months had the least variation.It was also found that degree of chromosomal number variations varied with melon varieties.