蛋白质微阵列技术及其在医学领域中的应用

Protein Microarrays and Their Medical Applications

蛋白质微阵列是生物芯片的一种 ,其主要优势在于应用平面上的有序排列的许多管、腔 (孔 )或各自独立的点来进行样本检测 ,使大量样本的平行分析成为可能。应用此技术可同时分析诸多蛋白质的生物化学活性、蛋白质与蛋白质间、蛋白质与DNA间、蛋白质与RNA间 ,以及蛋白质与配体间的相互作用 ,从而在临床诊断、药物研究、环境监测。

The microarrays have revolutionised biomedical experimentation and diagnostics,enabling ordered high throughput analysis.During the past decade,classic solid phase substrates,such as microtitre plates,membrane filters and microscopic slides,were turn into high density,chip like structure.The concept of the arrayed library was central to this development which now extends from DNA to protein.The availability of such protein microarrays would facilitate the simultaneous analysis of thousands of interactions within a single experiment.They can be utilized for massively parallel testing of protein function or recognized their target polypeptide in complex biological solution.This article will focus on the current stategies used to generate protein microarray and their applications in biological research,medicine and diagnostics.