摘要
目的 获取人幽门螺杆菌热休克蛋白60基因(Hsp60),并将它克隆到质粒 pET-22b(+)中进行核苷酸序列分析。方法 利用 PCR技术扩增 Hsp60,并将其定向插入 pET-22b(+)载体中,通过 DNA序列分析仪进行核苷酸分析。结果DNA序列分析表明,所克隆的Hsp60基因序列与GeneBank公布的一致。结论 获得了序列正确的Hsp60基因,为其重组表达及相关研究奠定了基础。
[Abstract] Objective To obtain the heat shock protein 60(Hsp60)gene of Helicobacter pylori and clone it into plasmid pET - 22b ( + ) for nucleotide sequencing. Methods Hsp60 gene was amplified by PCR,then inserted into pET- 22b( + ) vector and sequenced.Results The sequence of Hsp60 gene was in agreement with that reported in Genebank. Conclusion A confirmed Hsp60 gene has been obtained in this paper.The study laid a good foundation of the recombination,expression and research of the gene.
出处
《中国生物制品学杂志》
CAS
CSCD
2002年第2期69-71,共3页
Chinese Journal of Biologicals
基金
国家自然科学基金(39700007)
"863"计划专题(102-07-03-06)
军队"十五"医药卫生科研课题(01MA-132)资助项目