幽门螺杆菌热休克蛋白60基因的克隆及序列分析

Cloning and Sequencing of Heat Shock Protein 60 (Hsp60) Gene of Helio-bacter pylori

目的 获取人幽门螺杆菌热休克蛋白60基因（Hsp60），并将它克隆到质粒 pET－22b（+）中进行核苷酸序列分析。方法 利用 PCR技术扩增 Hsp60，并将其定向插入 pET－22b（＋）载体中，通过 DNA序列分析仪进行核苷酸分析。结果DNA序列分析表明，所克隆的Hsp60基因序列与GeneBank公布的一致。结论 获得了序列正确的Hsp60基因，为其重组表达及相关研究奠定了基础。

[Abstract] Objective To obtain the heat shock protein 60(Hsp60)gene of Helicobacter pylori and clone it into plasmid pET - 22b ( + ) for nucleotide sequencing. Methods Hsp60 gene was amplified by PCR,then inserted into pET- 22b( + ) vector and sequenced.Results The sequence of Hsp60 gene was in agreement with that reported in Genebank. Conclusion A confirmed Hsp60 gene has been obtained in this paper.The study laid a good foundation of the recombination,expression and research of the gene.