重组人干扰素γ的分子结构及其生物活性

Molecular Structure and Biological Activity of Recombinant Human IFN-γ

目的 了解重组人干扰素γ的分子结构及其生物活性。方法 采用细胞病变抑制法，以日本干扰素γ参考品为标准，计算国际单位。以4种不同方法检测相对分子质量。结果 比活性≥3．0×107 IU/mg蛋白，相对分子质量低于理论值16900。氨基酸分析结果只有129个氨基酸，少于应有数目。N端氨基酸序列分析结果，15－19个氨基酸与理论序列相符。结论 重组人干扰素γ C末端氨基酸有缺失，但不影响其生物活性。

[Abstract] Objective To study on the molecular structure and biological activity of recombinant human IFN-7. Methods The biological activity of IFN-7 manufactured by Shanghai Institute of Biological Products (SIBP) was detected by cytopathy inhibition and calculated into international unit using Japanese IFN-y reference as standard, and the relative molecular weight of it was detected by 4 methods. Results The specific activity of the IFN-γmanufactured by SIBP was not less than 3.0 ×107 IU/mg protein,and the relative molecular weight of it was lower than 16900. It consisted of only 129 amino acids,and the sequences of 15 to 19 amino acids at the N - teminal of it were in accord with those in theory. Conclusion The deletion of amino acids was observed at the C - tenninal of IFN-y manufactured by SEBP. However, the biological activity of the EFN-γhas not been influenced.