牛神经生长因子的研制

Preparation of Bovine Nerve Growth Factor

目的 寻找新的制备神经生长因子的材料和方法。方法 利用牛精液为原料，进行两次阳离子交换层析。结果 获得了纯的牛神经生长因子，相对分子质量为30000，比活性为400000BU/mg，FPLC检测结果为一个峰，电泳检测纯度大于95％。结论 可以从牛精液中制备神经生长因子。

[Abstract] Objective To develop a novel material and a new method for preparing bovine nerve growth factor. Methods The nerve growth factor was purified from bovine seminal plasma by a two - step cation exchange chromatography. Results The relative molecular weight, specific activity and electrophoretic purity of the purified bovine nerve growth factor were 30000,400000BU/rag and above 95% respectively, and a single peak was observed on the FPLC profile of it. Conclusion Nerve growth factor can be purified from bovine seminal plasma.