预防O139霍乱B亚单位/全菌体口服疫苗的研究

Study on Subunit B/Whole Cell Oral O139 Cholera Vaccine

目的 研制预防O139霍乱的B亚单位／全菌体口服疫苗。方法 应用重组霍乱毒素B亚单位及福尔马林灭活的O139霍乱弧菌全菌体，构建成B亚单位／全菌体疫苗，并进行安全性及免疫原性检测。结果 经连续3天大剂量灌喂小鼠后，未见体重下降、发病或死亡现象。全部小鼠均健康活存。以腹腔或口服免疫小鼠后，均能产生外周血或肠道局部高滴度的特异抗体。免疫小鼠对O139活菌攻击有良好的保护作用。结论B亚单位／全菌体口服疫苗在小鼠试验中安全性及免疫原性良好。

[Abstract] Objective To prepare oral subunit B/whole cell vaccine for preventing cholera caused by strain 0139.Methods The subunit B/ whole cell vaccine was prepared with the subunit B of recombinant cholera toxin and the 0139 strains of vibrio cholerae inactivated with formalin,and the safety and immunogenic-ity of it were studied. Results No bodyweight loss, morbidity or death were observed in the mice immunized with the oral vaccine at large dosages for 3 days. All the mice were survival and healthy. The specific antibody of high liters were detectecd out in the peripheral blood and intestinal tract of mice immunized with the vaccine by oral route or I. P. injection. The vaccine protected the mice against the challenge with live 0139 strain. Conclusion The subunit B/whole cell vaccine showed good safety and immunogenicity in mice.