应用U937细胞泡沫化模型检测人血高密度脂蛋白生物学功能

Detection of Biological Activity of Himan Serum High Density Lipoprotein by U937 Foaming Cell Model

目的 准确检测人血高密度脂蛋白（HDL）的生物学功能。方法 采用U937细胞泡沫化模型，检测HDL逆向转运细胞内胆固醇（Ch）的生理功能。用 Brad Ford方法检测蛋白质（Pro）含量，Ch试剂盒检测细胞内 Ch含量，并比较不同剂量HDL转移出细胞内Ch的量。结果 在一定的HDL浓度范围内相关性好（r≥0．98），重复性高（CV=6．l％），回收率为91．8％－110．5％。结论 该方法能更直观地反映HDL转运胆固醇的功能。且具有使用人源细胞、操作简便和易于推广应用的特点。

[Abstract] Objective To detect the biological activity of human serum high density lipoprotein correctly. Methods The physiological function of HDL stimulating cholesterol (Ch) efflux from cells were detected by U937 foaming cell model. Protein and cholesterol contents were detected by Brad Ford method and Ch kit respectively, and the amounts of Ch stimulated with HDL at different dosages were compared. Results The method showed good relativity (r≥.98) within certain limits of HDL concentration. The reproducibility and recovery of it were 6.1% and 91.8%-110.5% respectively. Conclusion The method can reflect the function of HDL transferring cholesterol from cells more directly. Using human - derived cells, the method is simple to handle and easy to be spread.