低氧预处理提高下丘脑细胞缺氧耐受性与Na^+、K^+电流的关系

RELATIONSHIP BETWEEN ENHANCED ANOXIC TOLERANCE INDUCED BY HYPOXIC PRECONDITIONING AND Na^+、K^+ CURRENTS IN CULTURED HYPOTHALAMIC CELLS

目的 :研究低氧预处理提高下丘脑细胞缺氧耐受性与Na+、K+电流的关系。方法 :采用培养的大鼠下丘脑神经元 ,用膜片钳全细胞记录技术 ,观察低氧预处理对其钠电流 (INa)、钾电流 (IK)的影响。结果 :①低氧预处理未明显改变INa,但增加了IK 幅值 ;②急性缺氧导致对照组细胞INa、IK 明显受抑制 ,而经过低氧预处理的细胞INa、IK 受抑制程度显著低于对照组。结论

Aim: To investigate the relationship between enhanced anoxic tolerance induced by hypoxic preconditioning and Na +?K + currents. Methods: After hypoxic preconditioning and acute anoxia the I Na ?I K were measured in cultured hypothalamic cells by patch-clamp whole cell recording technique. Results: The amplification of Na + currents did not been significantly changed,but the amplification of K + currents was increased in hypoxic preconditioning neurons; acute anoxia lead to the inhibition of Na +?K + currents in the two groups,while Na +?K + currents in non-preconditioned control group were inhibited severelier than hypoxic preconditioning group. Conclusion: It is presumed that the enhanced anoxia tolerance induced by hypoxic preconditioning may be related to the opening of K + channels.