SLE患者外周血淋巴细胞膜K(V)通道电流的变化及意义

CURRENT ALTERATION OF K(V) CHANNEL IN SLE PATIENT'S PERIPHERAL BLOOD LYMPHOCYTES AND IT'S SIGNIFICENT

目的 :探讨系统性红斑狼疮 (SLE)患者外周血淋巴细胞膜电压依赖性钾 [K(V) ]通道电流的变化及意义。方法 :采用膜片钳全细胞电流记录方法 ,记录了SLE患者外周血淋巴细胞膜K(V)通道电流。结果 :SLE患者淋巴细胞膜K(V)通道电流明显下降 ,正常人该通道电流幅度为 (2 5 8.6± 112 .5 )pA ,SLE患者的为 (139.4± 5 8.5 )pA(P <0 .0 5 )。通道的其它特性包括激活电压、失活特性、通道关闭动力学特性及药理学特性未见变化。结论 :SLE患者细胞免疫功能下降可能与淋巴细胞膜K(V)

Aim: To observe the property alteration of K(v) channel in SLE patient's peripheral blood lymphocyte and its significant. Methods: The patch-clamp technique was used to record the current of K(V) channel in SLE patient's peripheral lymphocyte. Results: The current amplitude of K(V) channel in the SLE patient's lymphocytes decreased, it was (258.6±112.5) pA in healthy people, but in SLE patient it was (139.4±58.5)pA(P<0.05). There was no other changes in the property of channel, include activation potential, inactivation property, channel closing kinetics and its pharmacological property. Conclusion: The decline of SLE patient's cell immunity may be related to the decrease of the amplitude of K(V) channel current.