SEN病毒中国株流行病学研究及克隆和序列分析

Prevalence Study and Gene Sequence Analysis of Chinese SEN Virus Isolates

为了解SEN病毒中国株的流行病学及基因序列 ,采集肝炎患者 (甲～戊型 )、非甲～非戊型肝炎患者、ALT升高的婴幼儿、尿毒症患者、静脉毒瘾者和健康体检人群的血清标本 ,用巢式PCR检测SEN病毒 ,结果他们的检出率依次为 13 3%、2 0 5 %、4 8%、5 8%、5 7%和 0。PCR获得了两种不同长度阳性片段。选取 5份阳性PCR产物进行纯化、连接及转化 ,获取重组克隆 ,命名为 pGCEM -SENVL(1、2、3)、pGEM -SENVS(1、2 ) ,进行DNA序列分析。结果与SEN病毒 (AX0 2 5 6 6 7)序列同源性分别为 87 7%、76 1%、88 2 %、72 7%和 78 8%。首次发现我国存在SEN病毒散发感染 ,并存在不同于SEN病毒 (AX0 2 5 6 6 7)的中国变异株 。

To investigate the prevalence and gene sequence of Chinese SEN virus(SENV), a nested polymerase chain reaction(PCR)assay was established to detect SENV in 495 samples.The positive rates of SENV in the samples of normal subjects,hepatitis non A-E,hepatitis A-E,ALT elevated infants,hemodialysis patients and intravenous drug users(IVDUS)were 0.0%,20.5%,13.3%,4.8%,5.8% and 5.7% respectively.Positive SENV PCR products revealed two kinds of fragments, the long and the short.5 postive PCR products were purified,ligated and transformed, the recombinant plasmids obtained were designated as pGEM-SENVL(1,2,3)and pGEM-SENVS(1,2),their DNA sequences were analyzed.Their nucleotide sequence homology to SENV(AX025667)were 87.7% of pGEM-SENVL(1),76.1% of pGEM-SENVL(2),88.2% of pGEM-SENVL(3),72.7% of pGEM-SENVS(1),and 78.8% pGEM-SENVS(2)respectivily.It is the first report in China that the scattered infection of SENV occurred in different Chinese populations.Also,there are variants of SENV in China that differed from SENV(AX025667)and two variant isolates may exist simultaneously in a same patient.