热带假丝酵母1230 POX4、POX5基因及其编码蛋白的研究

Analysis of POX4 and POX5 Gene Encoded Proteins of Candida tropicalis 1230

根据GenBank中热带假丝酵母菌株pk2 3 3 (ATCC 2 0 3 3 6 )POX4和POX5基因序列 ,设计了两对引物。通过PCR扩增 ,用一种简易的方法克隆了 1 2 3 0菌株中这两个基因。对POX4、POX5的测序表明 ,1 2 3 0菌株的POX5基因与pk2 3 3菌株的POX5基因存在菌株间的差异。这种差异对蛋白功能的影响有待进一步研究。对POX4和POX5编码的蛋白PXP4、PXP5进行Pfam分析和二级结构预测后 。

POX4 and POX5 were cloned from Candida tropicalis with a simple method.The sequencing results show differences of POX5 between strain 120 and strain pk233.The searching of these two sequences against the curreent collection of Pfam profile HMMS located regions that belong to known domain families.Secondary structure prediction shows that N-terminal secondary structure of PXP4 and PXP5 are homologous to that of pig liver medium-chain acyl-coA dehydrogenase.Thus,the N-terminal of PXP4 and PXP5 may be involved in FAD binding.