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限制和修饰系统LlaBIII在构建抗噬菌体菌株中的作用 被引量：2

The Resistance Conferred by the R/M System LlaBIII Against Bacteriophages
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摘要 LlaBIII,isolated from the naturally occurring plasmid pJW566 from Lactococcus lactis subsp.cremoris W56,encoded a restriction and modification (R/M) system. The plasmid pJK1 carrying the R/M system LlaBIII was transformed into L.lactis IL1403 with type I R/M system located on chromosome and the strain MG1614[pAW601] with AbiS gene on plasmid pAW601, respectively. The transformants obtained showed stacking resistance against bacteriophages. The plasmid pJK1 was transformed into industrial strains L.lactis SMQ86 and CHCC2281,the transformants showed the EOP of the bacteriophages decreased by 10 -3 and 10 -5, respectively.The results indicated that the R/M system LlaBIII could protect strains from bacteriophages in dairy fermentation. LlaBIII,isolated from the naturally occurring plasmid pJW566 from Lactococcus lactis subsp.cremoris W56,encoded a restriction and modification (R/M) system. The plasmid pJK1 carrying the R/M system LlaBIII was transformed into L.lactis IL1403 with type I R/M system located on chromosome and the strain MG1614[pAW601] with AbiS gene on plasmid pAW601, respectively. The transformants obtained showed stacking resistance against bacteriophages. The plasmid pJK1 was transformed into industrial strains L.lactis SMQ86 and CHCC2281,the transformants showed the EOP of the bacteriophages decreased by 10 -3 and 10 -5, respectively.The results indicated that the R/M system LlaBIII could protect strains from bacteriophages in dairy fermentation.
作者 孔健 Jytte Josephsen 马桂荣
机构地区 山东大学微生物技术国家重点实验室 丹麦皇家畜牧农业大学乳制品和食品科学系
出处 《微生物学报》 CAS CSCD 北大核心 2002年第2期246-250,共5页 Acta Microbiologica Sinica
关键词 乳酸乳球菌 噬菌体 限制修饰系统 乳制品 发酵 抗噬菌体菌株 Lactococcus lactis, Bacteriophages, Restriction and modification system, Dairy fermentation
分类号 Q936 [生物学—微生物学]
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同被引文献20

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微生物学报
2002年 第2期

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