蛇毒复合酶对人胃癌细胞的抑瘤研究

Experiment on Inhibitory Effect of Venin Complex Enzyme on Cell of Human Gastric Carcinoma

目的 观察蛇毒复合酶对人胃癌细胞 SGC-790 1的抑瘤作用及机理。 方法 采用体外试验、流式细胞术和细胞形态学检查方法 ,观察人胃癌细胞的生长抑制率 ,以及细胞周期和细胞形态的变化。 结果 蛇毒复合酶对人胃癌细胞有明显的抑瘤作用 ,2 4 h抑瘤率达 6 7.5 % ,接近 5 -Fu阳性对照组 ,并且具有明显的时效和量效关系。细胞镜检及涂片染色发现癌细胞胞膜破裂、胞质外溢、细胞坏死。流式细胞术检测蛇毒复合酶对 S期细胞有明显杀伤作用 ,同时阻滞 G0 / G1期细胞进入 S期。 结论 蛇毒复合酶对人胃癌细胞具有一定体外抑瘤作用 ,其作用机理与直接破坏细胞胞膜和干扰细胞增殖周期有关。

Objective The purpose of this study was to observe the inhibitory effect and mechanism of venin complex enzyme (VCE) on the cells of human gastric carcinoma (SGC-7901). Methods The experiment in vitro, flowing cytometry (FCM) and morphological examination were applied on the cells of SGC-7901 to observe the rate of inhibition and the aileration of cell form as well as cell cycle. Results There was a remarkable inhibitory effect on the SGC-7901 in VCE group with the rate of 67.5%(after cultivated for 24 hours) closing to that in the 5-Fu positive control group, and a close correlation between the inhibitory function of VCE and either drug concentration or drug duration of action.The observation of stained smears by microscopy showed cytomembrane ruptured, cytoplasm fluxed and cell necrosed. With analyzed by FCM, the VCE mostly killed the S stage cells, and blocked the G0/G1 phase cells developing to be G2/M phase cells. Conclusions It is concluded that the VCE could obviously inhibit the growth of SGC-7901 in vitro.The mechanism may be related with disrupting cytomembrane directly and interfering the cell proliferation cycle.