摘要
为了探讨雌激素作用于神经系统的机理,采用硫酸镍铵增强显色的免疫组化SP法研究了新的雌激素受体(ER-β)在成年雌雄大鼠脑内的分布。研究证实ER-β免疫阳性物质主要位于神经元的细胞核内,但在个别脑区也可在胞浆甚至突起内检测到。最强的ER-β免疫阳性信号见于前嗅核、大脑皮质、小脑浦肯野细胞、斜角带垂直部、蓝斑和三叉神经运动核等部位;中等强度的染色见于隔内侧核、杏仁外侧核、黑质、中央灰质等部位;较弱的阳性反应见于下丘脑与杏仁复合体的部分核团。在一些部位还存在表达水平甚至细胞内定位模式的性别差异,如前庭上核内的表达只见于雌性;雄性大鼠三叉神经运动核内ER-β蛋白主要表达于胞浆内,细胞核为阴性;而在雌性大鼠该部位ER-β蛋白主要位于细胞核等。以上结果表明ER-β蛋白在大鼠脑内分布广泛并具有一定的性别差异,在与学习记忆有关的脑区如大脑皮质和基底前脑内有很高的表达,提示在脑组织内雌激素可能通过ER-β这一新的信号途径发挥多种重要的调控作用,如学习记忆等。
The localization of the new estrogen receptor, ER-beta, in the rat brain was studied by immuno-cytochemical technique, and the results revealed that ER-beta immunoactive material was predominantly localized in the neuronal nucleus, but it was also detectable in the cytoplasm and neuronal processes. High levels of ER-beta immunopositive signals were detected in the cerebral cortex, vertical limb of 'the diagonal band, Purk-inje cells, locus ceruleus, and motor trigeminal,nucleus. Moderate levels were found in the medial septum, lateral amygdaloid nucleus, substantia nigra, and central gray. Weak signals were localized in some subregions of the hypothalamus and amygdaloid complex. Some differences of the expression of ER-beta immunoreactivity between male and female rats were also noticed. The above results provide the first detailed evidence that ER-beta protein is widely distributed in the rat brain, and ER-βmay be involved in some important brain function such as learning and memory.
出处
《实验生物学报》
CSCD
北大核心
2002年第1期15-20,共6页
Acta Biologiae Experimentalis Sinica
