PP_(333)及CCC对香椿试管苗增殖及生根移栽的影响

Effect of PP_(333) and CCC on growth multiplications and rooting of Toona sinensis in tissue culture

以 MS+6 - BA0 .2 mg· L- 1+GA1 .0 mg· L- 1为增殖基本培养基 ,分别附加不同浓度的 PP3 3 3 及 CCC,其中 1 0 mg·L- 1PP3 3 3 及 70 mg· L- 1CCC对香椿试管苗增殖生长有促进作用 ,尤以 1 0 mg· L- 1PP3 3 3 效果最好 ,同时可减轻玻璃化及愈伤组织发生 ;以 1 /2 MS+IBA1 .0 mg· L- 1为生根基本培养基 ,分别附加 0 .1 mg· L- 1PP3 3 3 及 1 0 mg· L- 1CCC,对试管苗生根壮苗有促进作用 ,而 1 0 mg·L- 1CCC最适宜 。

Added PP 333 or CCC at different concentrations to multiplying and rooting media when the shoots of Toona sinensis cultured.The basic medium for multiplication is MS+6-BA 0.2 mg·L -1+GA 1.0 mg.L -1,the basic media for rooting is 1/2 MS+IBA 1.0 mg·L -1.The results showed that PP 333(10 mg·L -1) or CCC(70 mg·L -1) improved the multiplication of shoots obviously,retarded the growth,the efficacy of PP 333(10 mg·L -1) is better.Meanwhile PP 333(0.1~0.3 mg·L -1) or CCC(10~70 mg·L -1) induced the callus and vitrification;PP 333 (0.1 mg·L -1) or CCC(10 mg·L -1) improved rooting rate and transplanting of the tube-plantlet,contrasted two kinds of hormons,CCC(10 mg·L -1) was evidently.After transplanting,the plant was short and strong,the colour of the leaves deepened,the rate of survitors was increased.