荧光原位杂交技术在植物细胞遗传学和绘制基因图谱中的应用现状与展望

Applications and prospects of fluorescence in situ hybridization on plant cytogenetics and gene mapping

荧光原位杂交是在分子水平上检测外源染色质的一种有效方法。其探针主要有染色体重复序列、总基因组 DNA、寡单拷贝序列和染色体涂色集中等 ,该技术在研究植物细胞遗传学、基因扩增、基因作图及植物进化和亲缘关系的鉴定上已广泛应用。

Fluorescence in situ hybridization (FISH) technique is a most effective method of detecting alien chromatin at molecular level.The main probes used in this technique are chromosomal repeat sequences,genomic DNA,oligo-or single copy sequence(s) and chromosome painting collections.It plays an increasing important role in a variety of research fields,such as plant cytogenetics,gene amplification,gene mapping,evolution and identification of the relationships of different species.In this paper,the related advances and future prospects of the application of FISH in plant cytogenetics and gene mapping are summarized.