摘要
A double haploid (DH) population of rice (Oryza sativa L.) derived from anther culture of ZYQ8/JX17, a typical indica and japonica hybrid, was used for genetic analysis of rice peduncle vascular system and panicle traits. The number of large vascular bundles (LVB), the number of small vascular bundles (SVB) in the peduncle, and the panicle traits including the number of primary rachis branches (PRB), the number of spikelets per panicle (SNP), peduncle top diameter (PTD), and panicle length (PL) were investigated in the parents and DH lines. The quantitative trait loci (QTLs) for each trait were analyzed based on the constructed molecular linkage map of this population. Three QTLs for LVB (qLVB_1, qLVB_6 and qLVB_7) were detected on chromosomes 1, 6, and 7, respectively. Two putative QTLs for SVB (qSVB_4 and qSVB_6) were mapped on chromosomes 4 and 6 respectively. Four QTLs (qPRB_4a, qPRB_4b, qPRB_6 and qPRB_7) on chromosomes 4, 6, and 7, respectively, were detected for PRB. Three QTLs (qSPN_4a, qSPN_4b and qSPN_6) were identified on chromosomes 4 and 6, respectively, which could significantly affect SPN. Five QTLs for PTD (qPTD_2, qPTD_5, qPTD_6, qPTD_8 and qPTD_12) were identified on chromosomes 2, 5, 6, 8, and 12, respectively. Three QTLs for PL (qPL_4, qPL_6 and qPL_8) were detected on chromosomes 4, 6, and 8, respectively. Clustering of QTLs, such as qLVB_6, qSVB_6, qSNP_6, qPTD_6, and qPL_6 detected in the interval G122_G1314b on chromosome 6, was found. These results suggest that some QTLs for peduncle vascular bundle system are possibly responsible for the panicle traits.
以籼稻 (OryzasativaL .ssp .indicaZYQ8)和粳稻 (O .sativassp .japonicaJX17)的杂交F1代花培加倍的DH群体为材料考察了该群体的穗颈节大小维管束数、一次枝梗数、每穗颖花数、穗颈节顶部直径和穗长 ,并用该群体构建的分子图谱进行数量性状座位 (QTL)分析。检测到控制大维管束的 3个QTL (qLVB_1、qLVB_6和qLVB_7)分别位于第 1、第 6和第 7染色体 ;控制小维管束的 2个QTL (qSVB_4和qSVB_6 )分别位于第 4和第 6染色体 ;控制一次枝梗的 4个QTL (qPRB_4a、qPRB_4b、qPRB_6和qPRB_7)分别位于第 4(2个 )、第 6和第 7染色体 ;每穗颖花数的 3个QTL (qSPN_4a、qSPN_4b和qSPN_6 )分别位于第 4(2个 )和第 6染色体上 ;穗颈节顶部直径的 5个QTL (qPTD_2、qPTD_5、qPTD_6、qPTD_8和qPTD_12 )分别位于第 2、第 5、第 6、第 8和第 12染色体 ;穗长的 3个QTL (qPL_4、qPL_6和qPL_8)分别位于第 4、第 6、第 8染色体上。其中qLVB_6、qSVB_6、qSPN_6、qPTD_6和qPL_6均位于第 6染色体的G12 2_G1314b之间 ;qPL_8和qPTD_8位于第 8染色体的GA40 8_BP12 7a之间 ;qPRB_4a和qSPN_4a位于第 4染色体的G177_CT2 0 6之间 ;qPL_4和qSPN_4b位于第 4染色体CT40 4_CT5 0 0之间 ;qSVB_4所在的区间与qPL_4、qSPN_4b和qPRB_4b所在的区间相邻。
